Rac1活性抗体
active rac1-gtp [26903]
[30 µl]
catalog number: 26903
gene symbol: rac1
description: anti-active rac1-gtp mouse monoclonal antibody
background: small gtpases are a super-family of cellular signaling regulators. rac belongs to the rho sub-family of gtpases that regulate cell motility, cell pision, and gene transcription. gtp binding increases the activity of rac, and the hydrolysis of gtp to gdp renders it inactive. gtp hydrolysis is aided by gtpase activating proteins (gaps), while exchange of gdp for gtp is facilitated by guanine nucleotide exchange factors (gefs).
immunogen: recombinant full length protein of active rac1
applications: ip, ihc
recommended dilutions: 1 µg for 1~2 mg total cellular proteins for ip;
1:100 dilution for ihc
concentration: 1 mg/ml
host: mouse
format: liquid
clonality: monoclonal
isotype: igm
purity: purified from ascites
storage buffer:
preservative: no
constituents: pbs (without mg2+ and ca2+), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity: anti-active rac1 antibody recognizes active rac1 from vertebrates.
storage conditions: store at -20°c. avoid freeze / thaw cycles
immunoprecipitation/western blot:
rac activation assay. mef cells were treated with (lane 2) or without (lane 1) pdgf. cell lysates were incubated with an anti-active rac monoclonal antibody (cat # 26903) (top panel). th precipitated active rac was immunoblotted with an anti-rac rabbit polyclonal antibody (cat # 21003). the bottom panel shows the western blot with anti-rac of the cell lysates used (5% of that used in the top panel).
immunofluorescence:
immunofluorescence for the active rac using anti-active rac1-gtp mouse monoclonal antibody [26903] shows rac-gtp immunolabeling (green) in combination with cofilin (red) on brain tissue sections.
the tissue sections were fixed with -20 ºc methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1m phosphate buffer with 0.3% triton x, and 4% bsa for 24h at room temperature. secondary antibodies were anti-mouse alexafluor488 and anti-rabbit alexafluor594 at 1:1000.
publications:
1.
cytoskeleton volume 71, issue 4, pages 257–272, april 2014
2.
neutrophil methods and protocols methods in molecular biology volume 1124, 2014, pp 251-268
3.
molecular and cellular neuroscience volume 60, may 2014, pages 10–25
4.
the journal of immunology april 14, 2014 1301155
5.
international journal of cancer volume 135, issue 11, pages 2493–2506, 1 december 2014
6.
j biol chem. 2014 may 30;289(22):15244-58
7.
mol cell biol. 2014 nov 15;34(22):4186-99
8.
plos one. 2014 sep 23;9(9):e108257
9.
cancer lett. 2015 jan 28;356(2 pt b):382-91
10.
physiological reportspublished 24 october 2014vol. 2no. e12175doi: 10.14814/phy2.12175
11.
anticancer research december 2014 vol. 34 no. 12 7111-7117
12.
cancer letters. march 1, 2015volume 358, issue 1, pages 76–84.
13.
integr biol (camb). 2014 dec 24
14.
mol neurobiol. 2015 jan 24
武汉纽斯特生物技术有限公司
18972339958
中国 武汉
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[30 µl]
catalog number: 26903
gene symbol: rac1
description: anti-active rac1-gtp mouse monoclonal antibody
background: small gtpases are a super-family of cellular signaling regulators. rac belongs to the rho sub-family of gtpases that regulate cell motility, cell pision, and gene transcription. gtp binding increases the activity of rac, and the hydrolysis of gtp to gdp renders it inactive. gtp hydrolysis is aided by gtpase activating proteins (gaps), while exchange of gdp for gtp is facilitated by guanine nucleotide exchange factors (gefs).
immunogen: recombinant full length protein of active rac1
applications: ip, ihc
recommended dilutions: 1 µg for 1~2 mg total cellular proteins for ip;
1:100 dilution for ihc
concentration: 1 mg/ml
host: mouse
format: liquid
clonality: monoclonal
isotype: igm
purity: purified from ascites
storage buffer:
preservative: no
constituents: pbs (without mg2+ and ca2+), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity: anti-active rac1 antibody recognizes active rac1 from vertebrates.
storage conditions: store at -20°c. avoid freeze / thaw cycles
immunoprecipitation/western blot:
rac activation assay. mef cells were treated with (lane 2) or without (lane 1) pdgf. cell lysates were incubated with an anti-active rac monoclonal antibody (cat # 26903) (top panel). th precipitated active rac was immunoblotted with an anti-rac rabbit polyclonal antibody (cat # 21003). the bottom panel shows the western blot with anti-rac of the cell lysates used (5% of that used in the top panel).
immunofluorescence:
immunofluorescence for the active rac using anti-active rac1-gtp mouse monoclonal antibody [26903] shows rac-gtp immunolabeling (green) in combination with cofilin (red) on brain tissue sections.
the tissue sections were fixed with -20 ºc methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1m phosphate buffer with 0.3% triton x, and 4% bsa for 24h at room temperature. secondary antibodies were anti-mouse alexafluor488 and anti-rabbit alexafluor594 at 1:1000.
publications:
1.
cytoskeleton volume 71, issue 4, pages 257–272, april 2014
2.
neutrophil methods and protocols methods in molecular biology volume 1124, 2014, pp 251-268
3.
molecular and cellular neuroscience volume 60, may 2014, pages 10–25
4.
the journal of immunology april 14, 2014 1301155
5.
international journal of cancer volume 135, issue 11, pages 2493–2506, 1 december 2014
6.
j biol chem. 2014 may 30;289(22):15244-58
7.
mol cell biol. 2014 nov 15;34(22):4186-99
8.
plos one. 2014 sep 23;9(9):e108257
9.
cancer lett. 2015 jan 28;356(2 pt b):382-91
10.
physiological reportspublished 24 october 2014vol. 2no. e12175doi: 10.14814/phy2.12175
11.
anticancer research december 2014 vol. 34 no. 12 7111-7117
12.
cancer letters. march 1, 2015volume 358, issue 1, pages 76–84.
13.
integr biol (camb). 2014 dec 24
14.
mol neurobiol. 2015 jan 24
武汉纽斯特生物技术有限公司
18972339958
中国 武汉
苏州钢结构加工 苏州异形钢楼梯报价 常州消防钢楼梯安装
厂家直销吸塑成型耳机套航空耳套液体硅胶皮耳套
安阳煎饼果子培训 经验丰富的是哪家？
教育行业成投资热选，优行教育前景更广阔！
陵城区异地搬家公司小知识，喜运搬家实在的价格
Rac1活性抗体
供应二手工业机器人ABB IRB1400焊接机器人
山西热干面加盟招商哪家好
9012-GCR21美国美商实快压力开关
胶南学化妆化妆师要注意什么问题事项？
来贺自动称重机维修
开个作文机构怎么样？俞禾作文拥有众多的忠实粉丝
闵行专业的合租房装修|二手房出租装修|出租房公寓装修
久雅地热地板代理条件是什么？
宁夏争做医疗“减负先锋”，“互联网+”大潮已至
木材水分计 纸水分计 sanko指定代理商KG-101
未来魔法校加盟费多少 需要投资多少钱
供应广东管件自动封口盖
生物质颗粒包装秤生产商--精湛技术源于细致
儿童体能拓展 淘气堡 厂家直销 整体策划