“恒远生物”产品文献：补益中药对运动性肾缺血再灌注损伤大鼠肾脏炎症因子
补益中药对运动性肾缺血再灌注损伤大鼠肾脏炎症因子及 ecm 表达的影响
郑孟君1 ，曹建民2 ，郭 娴2 ，周海涛3
(1． 江南大学 体育学院，江苏 无锡 214000;2． 北京体育大学 运动人体科学学院，北京 100084;
3． 北京联合大学 生物化学工程学院，北京 100023)
摘 要: 观察补益中药对运动性肾缺血再灌注损伤大鼠肾脏炎症因子及 ecm 表达的影响。方法:75 只 7 周龄雄性wistar 大鼠随机分为 4 组:安静对照组( c 组，12 只) 、一般训练组( m 组，12 只) 、过度训练组( om 组，24 只) 和补益中药 + 过度训练组( tm 组，24 只) ，m、om、tm 组进行 8 周 56 天的游泳训练。采用专业灌胃器每天灌胃 1 次，tm 组灌胃采用黄芪、红景天、丹参、苦参等组成的复合中药制剂，剂量为 1 g·kg － 1 ，体积为 5 ml·kg － 1 ，其他各组灌胃等量生理盐水。末次训练后 24 h，采用 pas 染色观察肾小球 ecm 沉积情况，免疫组织化学法检测肾组织 tnf-α、il- 1β、il-6、il-18 蛋白表达，rt-pcr 法检测肾组织 tnf-α mrna、il-1β mrna、il-6 mrna、il-18 mrna、tgf-β1 mrna表达。结果:1)8 周实验后，肾小球 ecm 沉积，c、m 组间无显著差异( p ＞ 0. 05 );om 组较 c、m 组显著增加( p ＜
0. 01);tm 组显著低于 om 组( p ＜ 0. 05)。2) 血尿素氮和血清肌酐水平，om 组和 tm 组显著高于 c 组( p ＜ 0. 01)， tm 组显著低于 om 组( p ＜ 0. 05);肾组织 tnf-α、il-1β、il-6 和 il-18 蛋白表达，om 组和 tm 组显著高于 c 组( p ＜
0. 01) ，tm 组显著低于 om 组( p ＜ 0. 05);肾组织 tnf-α mrna、il-1β mrna、il-6 mrna 和 il-18 mrna 表达，om、 tm 组显著高于 c 组( p ＜ 0. 01) ，tm 组显著低于 om 组( p ＜ 0. 05);肾组织 tgf-β1mrna 表达，tm 组( p ＜ 0. 05) 和 om 组( p ＜ 0. 01) 显著高于c 组，tm 组显著低于om 组( p ＜ 0. 05)。结论:8 周过度训练致大鼠发生运动性肾缺血再灌注，同时 ecm 沉积加强。补充补益中药可通过抑制肾脏组织 tnf-α、il-1β、il-6、il-18 的表达进而减轻了过度训练诱导肾脏缺血再灌注发生时肾脏组织 tgf-β1 的表达，抑制 ecm 的合成和( 或) 促进 ecm 的降解及组织病理学改变，延缓或避免过度训练导致的运动性缺血再灌注对肾脏的损害。
关键词: 补益中药;运动性肾缺血再灌注损伤;炎症因子;细胞外基质
中图分类号: g804． 7 文献标志码: a 文章编号: 1004 － 0560( 2015) 03 － 0068 － 06
effect of tonic medicine on kidney inflammation factors and ecm expression in rats of exercise-related renal ischemia-reperfusion injuries
zheng mengjun1 ，cao jianmin2 ，guo xian2 ，zhou haitao3
(1． sports college，jiangnan university ，wuxi 214000，jiangsu，china;
2. sport science college of beijing sport university ，beijing 100084，china;
3. biochemical engineering college of beijing union university ，beijing 100023，china)
abstract:objective:to study the protective effects of tonic medicine on kidney inflammation factors and extracellular ma- trix ( ecm ) expression in rats of exercise-related renal ischemia-reperfusion injuries． methods:75 male wistar rats at the age of 7 weeks were divided into 4 groups randomly:control group( c group，n = 12) ，general training group( m group，n =
12) ，overtraining group( om group，n = 24) ，tonic medicine + overtraining group( tm group，n = 24) ，the rats in m ， om ，tm groups were assigned to 8 weeks of swimming training，7 days a week． professional gavage were given once a day． the rats of tm group were given with compound chinese herbal medicine( the primary elements were chinese drugs for invigoration，such as astragalus，rhodiola rosea，salviae miltiorrhizae，sophora flavescens) ，gavages( ig) dose was 1 g
· kg － 1 ;ig volume was 5 ml·kg － 1 ，and other groups were given the normal saline． 24 hours after the last training，w e ob-
served the glomerular ecm deposition by the pas stain;detected the renal tissue of tnf-α，il-1β，il-6，il-18 protein by immunohistochemistry and detected the expression of tnf-α mrna，il-1β mrna，il-6 mrna，il-18 mrna，tgf-β1 mrna gene in renal tissue by rt-pcr method． results:1)8 weeks after the experiment，the glomerular ecm deposition of c group and m group appeared normal and showed no differences( p ＞ 0． 05);om group was significantly higher than that in c group and m group( p ＜ 0． 01) ，tm group was significantly lower than that of group om ( p ＜ 0． 05)． 2) om
收稿日期: 2015-03-04; 修回日期: 2015-03-29
基金项目: 国家体育总局课题(2013a101)。
作者简介: 郑孟君(1980—) ，女，讲师，硕士，主要研究方向为体育教学与运动训练。通信作者: 周海涛(1976—) ，男，副教授，硕士，主要研究方向为运动性疲劳与恢复。
第 3 期 郑孟君，等: 补益中药对运动性肾缺血再灌注损伤大鼠肾脏炎症因子及 ecm 表达的影响 ·69·
group and tm group blood urea nitrogen and serum creatinine were significantly higher than that of c group( p ＜ 0． 01)， tm group was significantly lower than that of group om ( p ＜ 0． 05);the expression of renal tnf-α，il-1 β，il-6 and il- 18 protein in om group and tm group was significantly higher than that in c group( p ＜ 0． 01) ，tm group was significant- ly lower than that of group om ( p ＜ 0． 05);the expression of renal tnf- α mrna，il-1 β mrna，il-6 and il-18 mrna in tm group and om group was significantly higher than that of c group( p ＜ 0． 01) ，tm group was lower than of om group( p ＜ 0． 05);the expression of renal tgf-β1mrna in tm group and om group was significantly higher than that of c group( tm ，p ＜ 0． 05;om ，p ＜ 0． 01) ，tm group was significantly lower than of om group( p ＜ 0． 05)． conclusion: 8 weeks overtraining caused the movement of rat renal ischemia reperfusion and increased the deposition of ecm ． tonic medicine can restrain the expression of renal tissue tgf-β1 during exercise-related renal ischemia-reperfusion injury in- duced by overtraining，w hich may be achieved by inhibiting the expression of il-1，tnf-α，il-6，il-18 in renal tissue． fi- nally it decreased the synthetic and increased the decomposition of ecm ，reduced the damage and the histopathological changes，as a result，tonic medicine can protect the rats from exercise-related renal ischemia-reperfusion injury．
key words:tonic medicine;exercise-related renal ischemia-reperfusion injury;inflammation factors;extracellular matrix
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“恒远生物”产品文献：补益中药对运动性肾缺血再灌注损伤大鼠肾脏炎症因子
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